Skin aging is a complex biological process influenced by a combination of endogenous or intrinsic and exogenous or extrinsic factors. numerous plants and plants extracts can attenuate degradation of skin matrix that can inhibit elastase, collagenase, and matrix metalloproteinases (mmps). polysaccharides, widely found in the animals, plants and microorganisms, are natural, non-toxic and biodegradable biopolymers which could be had anti-elastase, anti-collagenas activity to use in anti-ageing products. the aim of this study was to investigate the effect of anti-ageing potential of lactobacilli eps on normal human fibroblast and follow mmps gene expression level after their treatment.
Methods
Anti-elastase, anti-collagenase and antioxidant activity was determined for epss extracted from lactobacilli. mtt assay was performed on human normal fibroblast for cytotoxicity of epss. mmps gene expression level was determined by real time pcr for two selected eps.
Results
The result showed that extracted epss had high anti-collagenase, anti-elastase and antioxidant activity. most of them has no cytotoxicity for fibroblast and some of them promote cell proliferation. l. casei strain b9-1 and l. plantarum strain p35 were selected as the best strains. their eps showed significant effect on mmp1, 2, 3, 7, 9, 10, 12, timp1 and timp2 which are too important in skin photo-ageing.
Conclusion
Oral ingestion of probiotics i.e. lactobacilli exerts beneficial effects on the skin through mechanisms which are presumably initiated in the gut. alternatively, after topical application, probiotics may directly act on skin microflora. lactobacilli eps with high anti-collagenase, anti-elastase and antioxidant activity is a good option to use in anti-ageing products.
