Impact of gstp1 genetic polymorphisms on susceptibility risk of chronic myeloid leukemia

Fatemeh Ghadyani,1 Golale rostami,2 Mohammad hamid,3,* Hasan jalaeikhoo,4

1. Department of Cellular and Molecular , Faculty of Biology Sciences, Islamic Azad University of Tehran-North,Tehran, Iran
2. Department of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
3. Department of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
4. AJA Cancer Research Center (ACRC), AJA University of Medical Science, Tehran, Iran

Abstract

Introduction

Genetic variation influencing individual susceptibility to chemical carcinogens is one of the main factors leading to cancer development. associations between polymorphisms for genes encoding glutathione s-transferase (gst) enzymes involved in phase ii detoxification reactions and susceptibility to some cancers have been shown in several studies. we aimed at evaluating the role of gstp1 genetic polymorphisms in chronic myeloid leukemia (cml) patients in iranian population

Methods

In this study, we investigated the influence of inherited gstp1 (ile105val) gene polymorphism on the susceptibility to cml in iran in 104 cml patients and 102 healthy controls of matched age and sex using a pcr-rflp assay.

Results

The frequency of the gstp1 mutant allele was found to be 21.57% in cml patients and 23.08% in controls. however, no significant difference was found between controls and patients in terms of the gstp1 mutant allele frequency (p=0.8). no significant difference in genotype frequency was found between wild and mutant genotypes in cml patients when sex and smoking status were considered. with respect to clinical phase, cml patients in advanced phase (accelerated and blast crisis) had higher frequency of mutant homozygous (val/val) genotype (71.43%) compared to chronic phase (28.57%).

Conclusion

our results provide no evidence of a relationship between the gstp1 (iie105val) gene polymorphism and susceptibility to cml in iranian patients . the mutant allele may contribute significantly in the progression of disease from chronic to accelerated phase.

Keywords

Cml, polymorphism, gstp1, iranian population