To overcome difficulty of cancer treatment by conventional chemotherapy methods, the targeting drug delivery based on nano carriers has been developed, rapidly. poly [mpc-co-(bma)-co-(meonp)] (pmbn), as an amphiphilic nano polymer which could be targeted easily and solubilize hydrophobic agents, is so interested, nowadays. this polymer consists of three monomers. mpc (2-methacryloyloxyethyl phosphorylcholine) monomer simulates cell membrane of targeted cells to be able to fuses to its structure. bma (n-butyl methacrylate) unit let ability of loading all hydrophobic agents on polymer, and polymer could be combined with proteins due to meonp containing active ester groups.
Methods
For targeting leukemia stem cells overexpressing cd123 receptors, interleukin 3 ligand was conjugated to the polymer, and ganoderic acid derivative a as a fungal metabolite possessing acceptable anti-cancer properties, was incorporated to the polymer. after that, toxicity of designed carrier and polymer alone was analyzed.
Results
The efficiency of conjugation and loading were selected 72.1% and 77.5%, respectively. analysis of conjugated polymer, indicates that it is in a rod shaped with the size about 160±30 nm and zeta potential of -43.8 mv. mtt assay showed more than 50% enhancement of toxicity compared to ga-a singly.
Conclusion
These data demonstrate that pmbn-il3-ga is an anionic nanostructure material with acceptable size and structure which could be a proper remedy for cancer treatment. analysis of the nanostructure vehicle by cytotoxicity analysis revealed an increase in anti-proliferative effect of ga. incidentally, polymer is non-toxic and biocompatible, individually.
Keywords
Pmbn, anionic polymer, ga-a, interleukin 3, cancer therapy
